Choose an option 0. Lyophilized from a 0. Identification of maspin and SP as novel hypomethylation targets in pancreatic cancer using global gene expression profiling. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. We examined SP expression in normal human tissues by quantitative reverse transcription polymerase chain reaction and immunohistochemistry. Recent studies have shown that SP is highly expressed in both the cytoplasm and nucleus of cells in poorly differentiated bladder cancers.

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The best hybridomas were subcloned and frozen.

Anti-SP binding protein antibody (ab) | Abcam

From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Primary Antibody Dilution Buffer: Value indicates the geometric mean of the expression levels of three housekeeping genes used in the study. SP calcium-binding protein expression is associated with high-risk proliferative lesions of the breast. Expressed in brain, spleen, and lung. Am J Surg Pathol. Mass in vial mg ug ng. Optimal dilutions should be determined by each laboratory for each application.

Localization of SP protein in the human gut.


In panel F, the arrowheads indicate some positive nuclei in the esophageal epithelium. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, s100pp display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. Scrape cells off the plate and transfer to microcentrifuge tubes.

Gastric carcinomas showed the strongest immunoreactions, almost half of the specimens having strong signals.

In the body of the stomach, the immunostaining was localized to the mucus producing surface epithelial cells and chief cells of the gastric glands. Various segments of the intestine were also dissected for a more detailed immunohistochemical analysis. Recent studies have shown that SP is highly expressed in both the cytoplasm and nucleus of cells in poorly differentiated bladder cancers.

In the tumor categories studied, the expression of SP showed no correlation to the tumor grade data not shown.

Despite their relatively small size kDa and uncomplicated architecture, S proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation.

SP Antibody – Biocare Medical

Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. AW participated in real-time PCR and s100pp.


Preparing Cell Lysates Aspirate media. Pre-wash magnetic beads just prior to use: High stability is a prerequisite for a good biomarker. Tissue was stained using DAB brown and counterstained with hematoxylin blue.

Recently, it has been shown that SP can induce anchorage-independence of tumor cells in vitro and improve tumor growth in a xenograft model. All segments including duodenum S100lileum Bcecum C and colon D showed positive reactions in the enterocytes. Submit a review and receive an Amazon gift card.

The immunostaining method included the following steps: Caanon to add to vial ml ul. Be the first to review Human SP Antibody and earn rewards!

Human S100P Antibody

Sonicate on ice three times for 5 sec each. A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads. Over-expression of SP promoted tumorigenesis and metastasis in diverse cancer models.

The supernatant is the cell lysate.